Nucific Bio X4: Ingredients, Weight Loss And Comparison

As a certified nutritionist my clients often ask me for advice on losing weight. This often includes recommendations for probiotic supplements of which I have many years of experience testing.

In this review, I will analyse the ingredient profile, go over the side effects, cover some real reviews, teach you how to use the supplement and compare it to some other probiotic supplements I have tried.

Ingredients

The ingredients inside of Bio X4 contain a blend of 4-Billion CFU probiotic bacteria, 88mg digestive enzymes and a combined 266g weight management blend. 

This graphic below shows all of Bio X4's Ingredients and their amounts.

The Bio X4 probiotic ingredients include the following 5 bacteria strains.

• Lactobacillus Acidophilus
• Lactobacillus Rhamnosus
• Lactobacillus Plantarum
• Bifidobacterium Lactis
• Bifidobacterium Animalis

The Bio X4 digestive enzyme ingredients include the following 3 proteins. 

• Amylase
• Bromelain
• Lipase

The Bio X4 weight loss blend ingredients are made up of the following 2 extracts.

• Green Tea
• Caralluma Fimbriata

Below I will analyze various clinical studies that have tested the Bio X4 ingredients for weight loss in humans to figure out if bio x4 can help you lose weight or not.

1. Lactobacillus Acidophilus Helps With Weight Reduction

A Randomized controlled study published in March, 2020 in the Scientific Reports, Peer Reviewed Journal DR Michael showed that Lactobacillus Acidophilus resulted in significant weight reduction in humans.

Upon completion of the trial, it was confirmed that the administered probiotic bacteria successfully withstood the presence of bile acids within the digestive system. However, cholesterol levels and plasma lipids remained unaltered. Notably, after a span of six months, significant reductions were observed in body weight, BMI, waist circumference, and waist-to-height ratio among the participants.

The trial enlisted 220 participants residing in Bulgaria, who possessed a body mass index (BMI) ranging from 25 to 34.9 kg/m2. Prior to their participation, these individuals provided written consent and received compensation for their involvement. The probiotic strains used were the same Bio X4 probiotic blend I listed previously.

2. Lactobacillus Rhamnosus Achieves Weight Loss

In 2004, a randomized controlled trial was conducted and subsequently published in the peer-reviewed British Journal of Nutrition by M Sanchez found Lactobacillus Rhamnosus CGMCC1.3724 probiotic bacteria helped obese women achieve sustainable weight loss in humans.

The trial spanned a duration of 24 weeks, during which participants were assigned to either an undisclosed placebo group or a group instructed to consume two capsules of a probiotic supplement containing 1.6 million colony-forming units (CFUs). This probiotic formulation included Bio X4 L. Rhamnosus bacteria and oligofructose/inulin prebiotic bacteria, to be taken once daily.

Based on the study's findings, it was concluded that the effects of Lactobacillus rhamnosus bacteria took time to manifest in women, and successful weight management necessitated both the consumption of the probiotic supplement and calorie restriction. The reason for the differential response to Lactobacillus rhamnosus bacteria between men and women in this study remains unclear.

3. Lactobacillus Plantarum Does Not Help With Weight Loss

A 2022 South-Korean study published in the Frontiers In Endocrinology authored by M Sohn, looked at the efficacy & safety of a 4-billion CFU probiotic containing gram-positive Lactobacillus Plantarum K50 bacteria. The study showed that L. Plantarum did not help with weight loss in humans.

Through a randomized, double-blind, placebo-controlled, clinical trial they tested the bacteria containing 81 South Koreans with a 25–30 kg/m2 BMI for a short period of 12 weeks.

To confirm if the bacteria had any impact it was decided that fat mass, anthropometric parameters, cholesterol levels, and other biomarkers for obesity would all be measured before and after.

At the end of the trial, it was noted that abdominal fat was unchanged in probiotic or placebo groups and there was no noted weight loss. One interesting finding however was that total cholesterol levels dropped in the probiotic group, and so did triglyceride levels and also leptin levels.

Another 2022 study authored by Y. Ma can reduce the body weight of mice fed high-fat diet but Bio X4 is a probiotic brand for humans and not for animals like mice. 

4. Bifidobacterium Lactis Does Not Reduce Body Fat

A 2021 published, randomized controlled trial from the University of Medical Sciences in Poznan, Poland, authored by D Czajeczny showed no significant changes in body fat were observed in either the placebo or Bifidobacterium lactis BS01 group. 

This trial investigated the effects of both Bifidobacterium lactis BS01 and Lactobacillus acidophilus LA02 on a cohort of 38 females aged between 19 and 33 years over a period of 6 months. Participants were randomly assigned to either receive daily supplementation of the probiotics or a placebo.

Various metrics including body mass, BMI, arm skinfold fat, body fat percentage, and waist circumference were assessed at the beginning and end of the trial.

5. Bifidobacterium Animalis Reduces Abdominal Fat

A 2016 study authored by Shota Takahashi noted that versus placebo, Bifidobacterium Animalis probiotic bacteria did reduce abdominal visceral fat but did not reduce the subcutaneous fat area or total body weight. The bacteria were able to increase mucin secretion & IgA production which was thought to help with intestinal barrier function.

The study tested the efficacy of Bifidobacterium Animalis ssp. Lactis GCL2505 on "visceral fat accumulation" in Japanese adults with a BMI 23–30 kg/m2 which by World Health Organization standards categorized them as overweight or obese. Other than that blood & urine tests showed them to be in good health.

The study instructed participants to avoid taking any other probiotic foods during the 12-week trial and instead take an 8-Billion CFU fermented milk drink containing only B. Lactis GCL2505 bacteria.

6. Amylase May Help With Fat Loss

Amylase is an enzyme that facilitates the breakdown of complex carbohydrates into simpler sugars. Supplementing with an amylase may help with weight loss and fat loss according to a "Systematic Review and Meta-Analysis of a Proprietary Alpha-Amylase Inhibitor from White Bean."

7. Bromelain Hasn't Been Studied For Weight Loss

Bromelain is a proteolytic enzyme found in pineapple that aids in the digestion of proteins. This enzyme may help metabolise proteins that could help cut down fat but presently there are no studies on Bromelain for weight loss.

8. Lipase Can Cause Weight Gain

Lipase is an enzyme responsible for the breakdown of fats and triglycerides into smaller molecules, such as fatty acids and glycerol. According to Mount Sinai the New York medical hospital, Lipase can induce modest weight gain compared to placebo groups.

9. Green Tea Extract High Dose Triggers Weight Loss

Green tea extract is a concentrated form of beneficial compounds derived from green tea leaves, known for its potential health-promoting properties and antioxidant effects.

One randomized clinical controlled trial from the National Yang-Ming University, Taiwan authored by IJ Chen showed that 856.8 mg of Green Tea Extract taken daily resulted in significant weight loss.

1 capsule of Bio X4 contains a low 100mg dose of green tea extract so it is unlikely to mimic these weight loss results.

10. Caralluma Fimbriata Extract Helps With Appetite Control

Caralluma fimbriata extract is a concentrated form of a natural plant extract derived from the Caralluma fimbriata cactus.

A 2021 study by the University of Sydney & University of Queensland and authored by Amanda Rao (PhD) found that Caralluma Fimbriata extract taken daily for 16 weeks may help maintain body weight and help with appetite control in obese adults.

May Support Weight Loss

After analysing Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium lactis, amylase, bromelain, lipase, green tea extract, and caralluma fimbriata extract the ingredients inside of Bio X4 I can conclude that it may help with supporting weight loss.

The effectiveness of Bio X4 in promoting weight loss varies depending on the specific ingredient. Lactobacillus acidophilus has shown significant weight reduction effects in humans, while Lactobacillus rhamnosus has demonstrated sustainable weight loss in obese women. However, Lactobacillus plantarum did not contribute to weight loss in the studied population.

Bifidobacterium lactis did not lead to significant changes in body fat, and the effects of Bifidobacterium animalis were observed in reducing abdominal visceral fat specifically. Amylase, an enzyme that aids in carbohydrate breakdown, may help with weight loss. Bromelain, lipase, and green tea extract have not been extensively studied for their direct effects on weight loss.

Caralluma fimbriata extract has shown potential in helping with appetite control and maintaining body weight in obese adults.

Therefore, while some ingredients in Bio X4 may have shown positive effects on weight loss or appetite control in specific studies, the overall impact of Bio X4 on weight loss cannot be determined without further research specifically targeting the combination of ingredients in the product.

Expect Minor Side Effects

Taking Bio X4 can result in minor side effects for some people. Whilst the probiotic supplement is safe for adults some side effects include diarrhea, minor bloating, and stomach pain.

This happens when people first start to include probiotics in their diet and their gut adjusts to the new bacteria strains. 

Users may however then experience relief from diarrhea once the microbiome settles and adjusts to the 5 Bio X4 probiotic bacteria strains.

Can Bio x4 Raise Blood Pressure?

The caffeinated green tea content inside of Bio X4 can actually significantly lower systolic blood pressure according to a study called "Effect of green tea supplementation on blood pressure" authored by Renfan Xu and published in Medicine (Baltimore).

It Has A lot Of Reviews

Nucific 4-in-1 weight management has over 8,900 customer reviews on amazon with a 3.8 out of 5 star rating on Amazon which isn't great but not terrible either.

One customer in 2020 called Terri La Rue claimed that they have celiac disease and that she usually only had one bowel movement every 3 to 5 days. Since taking Bio X4 she now has one every day. Most of the 5 star reviews are similar in that they do not mention anything about weight loss.

In 2020 a Bio X4 customer called Joe Iverson hilariously shared that they were still fat whilst deciding to give the supplement 1 star out of a possible 5. It's unclear how long Joe took the product for and if they also did anything else to help aid in potential weight loss like exercise for example.

Take It 3 Times Per Day

Nucific advise that you will need to take one capsule with each meal, three times a day, and you can take up to two per meal so a maximum of 6 capsules per day.

Bearing in mind that you will get 90 capsules per jar this means you will have to buy at least one jar per month. They also recommend taking Bio X4 before your meals for optimal digestive health.

It Will Expire

Yes, Nucific Bio X4 does have an expiry date. The probiotic blend inside of Bio X4 will start to expire after around 1 year of the original manufacture date. At this point, the probiotic bacteria will start to die and not have any positive effect. Look at the bottom of your bottle to see the manufacture and expiry dates.

Only Available On Their Website

Nucific sell Bio X4 online on their website, where you can take advantage of discounted offers. It’s actually not available elsewhere at the moment.

One 30-day supply i.e. a jar of 90 capsules is priced at $99 - extremely expensive compared to other probiotic supplements.

However, you can get a discount for buying multiples: save 11% with 3 jars ($261.36) or 18% with 6 jars ($487.08).

Bio X4 has a 90 day money back guarantee so there is quite a bit of faith behind the product and you may ask for your money back if you’re unhappy with the results.

You are however responsible for shipping charges with the refund only processed once they receive the product at their facility. To start the refund process simply call 888-679-5520 from Mon-Fri 5AM-7PM PST or Sat-Sun 6AM-4:30PM PST.

Comparing To Other Products

Bio X4 and Total Restore are both probiotics I have tried that claim to support digestive health and overall wellness. However, Bio X4 focuses on providing probiotics, prebiotics, and digestive enzymes.

Total Restore contains a blend of ingredients such as glutamine, licorice root extract, and marshmallow root that are intended to soothe the digestive tract and support gut health.

Ultimately, the choice between these two supplements will depend on an individual's specific needs and preferences. Another probiotic like 1MD Platinum could be better if you are interested in weight management because of it's Lactobacillus Gasseri strain that may aid the metabolism.